Do dendritic cells express CD11b?

The CD8+ dendritic cell subset CD8+ cDCs express no or low levels of the integrin CD11b and other macrophage markers (Table 1).

What is CD123 a marker for?

Furthermore, CD123 is an important marker for the identification and targeting of LSCs for refractory or relapsed leukemia.

Is Ly6C a surface marker?

Mouse blood monocytes include two main subsets usually discriminated by the expression of the Ly6C surface marker.

What is CD3 -/ CD16 CD56 +?

CD3-CD16+CD56+ are cytotoxic lymphoctyes also known as Natural Killer (NK) cells. NK lymphocytes destroy cells that are infected by viruses, cancer cells, or cells that are subject to rejection after tissue transplantation.

Is CD11b a marker for neutrophils?

CD11b is an immunological marker for early detection of neonatal sepsis [29] and a cell surface antigen of neutrophil [30]. Its expression on neutrophil cell surface, however, increases substantially within a few minutes after the cell comes into contact with bacteria or endotoxins [31].

Is CD11b a macrophage marker?

The CD11 protein is actually a heterodimer complex that consists of CD11b and CD18. CD11 is involved in numerous adhesion-related associations between cells such as monocytes, macrophages, natural killer (NK) cells, and granulocytes.

Where is CD123 expressed?

CD123 is expressed widely in hematological malignancies including acute leukemia, blastic plasmocytoid dendritic cell neoplasm (BPDCN), and hairy cell leukemia (11, 12). Most previous investigations elucidated the role of CD123 in acute myelogenous leukemia (AML).

Are eosinophils CD11b positive?

Eosinophils have been reported to express β2 (CD11a, CD11b and CD18) integrins on their surface, and the levels of CD11b on eosinophils are enhanced by eosinophil-activating cytokines such as IL-5. In this study, expression of CD11b on eosinophils was analyzed by flow cytometry.

What is gr1 antibody?

Gr-1 is expressed on mature granulocytes in bone marrow and peripheral tissues. The Anti-Gr-1 antibody also stains monocytes transiently during their differentiation in bone marrow and at low levels plasmacytoid dendritic cells in lymphoid tissues.

What is a traditional flow cytometer?

Traditional flow cytometers consist of three systems: fluidics, optics and electronics. The fluidics system consists of sheath fluid (usually a buffered saline solution) that is pressurized to deliver and focus the sample to the laser intercept or interrogation point where the sample is analyzed.

Can flow cytometry identify immune cells?

Immune Cell Characterization by Flow Cytometry Flow cytometry is a powerful technique that is widely used to identify and characterize different immune cell types in heterogeneous samples.

How does flow cytometry detect apoptosis?

The detection of apoptosis by flow cytometry utilizes multiple targets along the cascade of events associated with apoptosis.

What are the challenges of multi parameter flow cytometry?

Spectral Analyzers One of the challenges of multi-parameter flow cytometry is compensation (or erasing spectral overlap) between flurochromes. A new type of flow cytometer, the spectral analyzer is specifically designed to address this problem.