What is the size of BSA?

7.1 nm
According to the literature, BSA has a nominal size of 7.1 nm and a molecular mass of 66.5 kDa. Bovine serum albumin shows a natural tendency to dimerize under stress conditions, with BSA dimers displaying a molecular mass of 132 kDa.

What is BSA pi?

Discussion. The BSA isoelectric point is between pH 4.5–4.8; it is the pH at which the molecule net charge becomes zero. Near the isoelectric point, the BSA molecules have less inter-molecule electrostatic repulsion.

Why is BSA called Fraction V?

BSA is often referred to as “Fraction V”, based on the original fractionation of human plasma developed by Edwin Cohn. Primarily developed for human albumin production, the process was later adapted to bovine serum albumin.

What is a BSA standard?

Bovine Serum Albumin (BSA) Standard, is a universally known protein standard utilized in different protein assays for standard curves. Our BSA Standard is a high-quality reference sample for assays. To ensure accurate results, it is prepared with extra care for reproducible results.

Why is BSA used for standard curve?

The bovine protein standard is the preferred standard in protein assays because in addition to its ability to increase signal in assays, bovine serum is affordable and easily mass-producible.

How do you calculate BSA?

Here are the formulas: Du Bois: BSA = 0.007184 * weight0.425 * height. Mosteller: BSA (m²) = SQRT{ [ height (cm) x weight (kg)] / 3600 }

How do you make a standard BSA curve?

Mix 50 μl of the BSA standard solution (2 mg/ml) with 950 μl of diluent and mix well to prepare a 0.1 mg/ml BSA standard solution. Prepare 2 sets of the dilutions of BSA standard solution as shown below 1.5 ml microtubes (7 types x 2 sets = 14 microtubes).

What is the concentration of BSA?

The concentration of BSA in solution can be determined by substituting the molecular weight, extinction coefficient and λ max into a derived form of the Beer – Lambert Law. A substance’s λmax is the wavelength at which it experiences the strongest absorbance. For BSA, this wavelength is 280 nm.

What are BSA standards?

Bovine Serum Albumin (BSA) is the standard reference for total protein quantitation by colorimetric assays. Expedeon BSA standards are designed for serial dilution to generate accurate standard curves and are precisely formulated at 2mg/ml.

How do you calculate BSA for children?

We retrospectively compared body surface area (BSA) using the Mosteller formula square root(H x (w/360)) and a simple weight-based BSA formula ([4Wkg+7]/ [90+Wkg]).

What is normal BSA range?

The overall BSA was 2.04 +/- 0.24 m(2): 1.81 +/- 0.19 m(2) in normal-weight, 1.99 +/- 0.16 m(2) in overweight, and 2.21 +/- 0.22 m(2) in obese subjects. These values were significantly higher in overweight and obese patients compared with the values using the DuBois-DuBois formula (overall, 2.00 +/- 0.22 m(2), P < .

What is a BSA curve?

This standard curve of Protein concentration vs absorbance used to estimate protein concentrations from samples 21-56. R 2 =0.989; coefficient of the slope b= 0.422, and intercept = 0.0062 was used to calculate protein concentration in mg/ml from absorbance readings of egg samples.

How do you calculate BSA concentration?

What is BSA of a child?

Body surface area (BSA) is an important index of body size in both adults and children. The use of BSA allows for standardization of the important physiological parameters such as cardiac function, glomerular filtration rate and proteinuria, which are indexed by BSA.

What is normal range for BSA?

What is BSA formula?

According to Mosteller’s “simplified calculation of body-surface area In metric terms” the body surface area = the square root of product of the weight in kg times the height in cm divided by 3600.

How do you calculate BSA standards?

How do you calculate the BSA of a child?

We retrospectively compared body surface area (BSA) using the Mosteller formula square root(H x (w/360)) and a simple weight-based BSA formula ([4Wkg+7]/ [90+Wkg]). The participants were 363 children who underwent cardiac surgery from 1991 to 2000.

What is the hydrodynamic radius of BSA?

The dependences of the hydrodynamic radius ( R h) of BSA aggregates on the portion of aggregated protein (γ agg) calculated at different initial concentrations of the protein: 0.50 (1), 1.00 (2), 1.50 (3) and 1.75 mg∙mL −1 (4).

What is hydrodynamic radius in biophysics?

Note that in biophysics, hydrodynamic radius refers to the Stokes radius, or commonly to the apparent Stokes radius obtained from size exclusion chromatography. arises in the study of the dynamic properties of polymers moving in a solvent.

What is the kinetics of heat-induced aggregation of BSA?

The kinetics of heat-induced aggregation of BSA was studied in 0.1 M Na-phosphate buffer, pH 7.0. The buffer was placed in a cylindrical cell with the internal diameter of 6.3 mm and pre-incubated for 5 min at a designed temperature (70 °C).

What is the process of BSA aggregation at 70 °C?

On the basis of analysis of the kinetic data for BSA aggregation at 70 °C the following scheme of the aggregation process can be proposed (Fig. 8 ). The fast stage of protein unfolding (stage 1; M N → M U) is followed by the slow dimerization of the unfolded protein molecules with the rate constant of the second order k II (stage 2).