What causes platelet Satellitism?

Platelet satellitism, which is an in vitro phenomenon, is associated with EDTA-treated blood at room temperature. It is seen in patients with vasculitis, lupus, mantle cell lymphoma, and marginal zone B-cell lymphoma, and most commonly in healthy individuals.

Which anticoagulant is usually implicated in platelet Satellitism?

In vitro grouping of the platelets around polymorphonuclear neutrophils (PMNs) and other cells imparting a rosette-like appearance is a phenomenon popularly called “platelet satellitism”, and it occurs in blood samples anticoagulated only with K3EDTA at room temperature.

What is platelet Satellitosis?

Platelet satellitism (PS) is a rare phenomenon described sporadically in the literature. It involves adhesion of thrombocytes sensitized by antibodies to leucocytes and is observed in vitro in EDTA anticoagulated blood.

Where do platelets adhere in platelet Satellitism?

Platelet satellitism (PS) is an uncommon and unique phenomenon observed on peripheral blood smears. It is defined as adherence of platelets on the surface of polymorphonuclear leucocytes and imparting a rosette-like appearance.

What is platelet Satellitism does it affect platelet count using this technique?

Platelet satellitism and platelet clumping can cause pseudo-thrombocytopenia. Platelet satellitism was first reported in the early 1960s. It is a rare condition that occurs when an IgG antibody forms in the presence of EDTA, the anticoagulant that is used for the collection of hematology blood specimens.

What causes Pseudothrombocytopenia?

Pseudothrombocytopenia is caused by platelet clumping in vitro, which may be induced either by antibody‐mediated agglutination, the most important causes of which are ethylene‐diamine‐tetra‐acetic acid (EDTA)‐dependent agglutination and platelet satellitism, or aggregation secondary to platelet activation resulting …

What causes pseudothrombocytopenia?

What causes platelet dysfunction?

Platelet function disorders can develop or be inherited. The most common reason for someone to develop a platelet function disorder is from taking medication. Medicines such as ibuprofen or aspirin can affect platelet function. People with some liver or kidney problems may develop a platelet function disorder.

What causes EDTA induced pseudothrombocytopenia?

EDTA-induced pseudothrombocytopenia, the most frequently seen form in clinical practice, occurs mainly due to reaction of antiplatelet antibodies including cold agglutinin type of IgG, IgM and IgA with platelet surface antigens.

Which is the most common cause of acquired platelet dysfunction?

Acquired platelet dysfunction, which is common, may result from aspirin, other nonsteroidal anti-inflammatory drugs (NSAIDs), or systemic disorders.

What can affect platelet function?

The most common drugs that affect platelet function are aspirin and other nonsteroidal anti-inflammatory drugs (NSAIDs), along with antiplatelet drugs such as clopidogrel and similar drugs that are used to prevent strokes and heart attacks.

What is EDTA-induced pseudothrombocytopenia?

EDTA-dependent pseudothrombocytopenia (PTCP) is the phenomenon of a spurious low platelet count due to EDTA-induced aggregation of platelets. Since the failure to recognize EDTA-dependent PTCP may result in incorrect diagnosis and inappropriate treatment, the recognition of this phenomenon is very important.

What is acquired platelet dysfunction?

Acquired platelet function defects are conditions that prevent clotting elements in the blood called platelets from working as they should. The term acquired means these conditions are not present at birth. Blood clotting normally occurs when there is damage to a blood vessel.

What causes low platelet function?

Factors that can decrease platelet production include: Leukemia and other cancers. Some types of anemia. Viral infections, such as hepatitis C or HIV.

What causes thrombocytopenic disorder?

Thrombocytopenia might occur as a result of a bone marrow disorder such as leukemia or an immune system problem. Or it can be a side effect of taking certain medications. It affects both children and adults.

What deficiency causes low platelets?

Vitamin B-12 A deficiency of B-12 has been associated with low platelet counts. The best sources of vitamin B-12 tend to be animal-based foods, such as: beef liver. clams.

What causes EDTA-induced pseudothrombocytopenia?

What causes platelets to be normal in vivo?

In vivo and in vitro platelet functions were normal. Platelet satellitism is an in vitro phenomenon, the cause of which is unknown. We are unable to relate it to functional abnormalitles of the blood, the clinical condition of the patient, or to drugs.

What is the clinical significance of a false low automated platelet count?

The main clinical significance of this event lies in false low automated platelet counts (pseudothrombocytopenia) and highlights the importance of peripheral blood smear analysis in patients with thrombocytopenia.

What causes platelet adhesion to neutrophils?

Adhesion of platelets to neutrophils is possible by existence of an active stimulus presented on the surface of platelets (11, 12, 17).

What does a platelet count of 35 10 9 mean?

However, routine blood count showed a platelet count of 35 × 10 9 /L with a normal hemoglobin and white blood cell count. He was referred to hematology for evaluation of thrombocytopenia. Physical examination was unremarkable.