What type of end is produced by cleavage with HindIII?

With HindII, cleavage occurred in the middle of a six-base-pair recognition site, yielding what are known as blunt-end fragments (see Figure 3, in which PvuII similarly produces blunt-end fragments).

What is the site for restriction enzyme cleavage?

Generally, the cleavage sites are symmetrically positioned, or palindromic. Restriction enzymes can create fragments with sticky ends, as is the case with the enzyme BamHI, or blunt ends, as with HaeIII (Table 8.1). Double bars indicate the cleavage site in the DNA strand.

Where does the HindIII enzyme cut?

HindIII (pronounced “Hin D Three”) is a type II site-specific deoxyribonuclease restriction enzyme isolated from Haemophilus influenzae that cleaves the DNA palindromic sequence AAGCTT in the presence of the cofactor Mg2+ via hydrolysis.

Does Hind 3 produce blunt ends?

Option B: Hind 3: It is a type 2 restriction endonuclease which gives sticky ends.

What type of ends are produced by Hind 3?

Option B: Hind 3: It is a type 2 restriction endonuclease which gives sticky ends. It is isolated from Haemophilus influenzae.

What is the recognition site of Hind 2?

Hind II was the first discovered restriction endonuclease enzyme. It has been isolated from Haemophilus influenzae Rd. It cuts DNA molecules at a particular point by recognising a specific sequence of six base pairs. This specific sequence is known as the recognition sequence for Hin d II.

What is the structure of HindIII?

The structure of HindIII is complex, and consists of a homodimer. Like other type II restriction endonucleases, it is believed to contain a common structural core comprising four β-sheets and a single α-helix.

What can we learn from HindIII restriction endonuclease site-directed mutagenesis?

Despite the uncertainty concerning the structure-catalysis relationship of type II endonucleases, site-directed mutagenesis of the restriction endonuclease HindIII has provided much insight into the key amino acid residues involved.

What is the mechanism of action of HindIII?

However, it is believed that HindIII utilizes a common mechanism of recognition and catalysis of DNA found in other type II enzymes such as EcoRI, BamHI, and BglII. These enzymes contain the amino acid sequence motif PD-(D/E)XK to coordinate Mg2+, a cation required to cleave DNA in most type II restriction endonucleases.

Where can I find more details about BSA-free HindIII?

Find more details at www.neb.com/BSA-free. HindIII has a High Fidelity version HindIII-HF™ ( NEB #R3104 ). High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing.