Can restriction enzymes cleave RNA?

Surprisingly, some restriction enzymes including AvaII, BanI and TaqI could cleave one or both strands of RNA/DNA heteroduplexes.

Are oligonucleotides DNA or RNA?

Oligonucleotides, or oligos, are short single strands of synthetic DNA or RNA that serve as the starting point for many molecular biology and synthetic biology applications! From genetic testing to forensic research and next-generation sequencing, an oligo may very well be the starting point.

Are oligonucleotides RNA?

Oligonucleotides are short DNA or RNA molecules, oligomers, that have a wide range of applications in genetic testing, research, and forensics.

Are oligonucleotides soluble in water?

A short DNA oligonucleotide (21-mer) is soluble in nearly anhydrous solutions of glycerol, ethylene glycol, formamide, methanol, and DMSO in the presence of a small amount of water (e.g., 99 % organic solvent and 1 % water) (Bonner and Klibanov 2000).

What types of RNA do we offer for oligonucleotides?

Single stranded RNA such and 2’O-Methyl-RNA for your shRNA, aptamers or chimerics as well as double-stranded siRNA. In addition to offering small scale oligonucleotides synthesis, we offer large scale oligos and tools that facilitate the design and usage of your oligo.

How are oligonucleotides synthesized?

The oligonucleotides are synthesised in 3′ to 5′ direction while attached covalently to a solid support (solid phase synthesis).The building blocks used for synthesis are DNA phosphoramidite nucleosides which are modified with different protection groups.

How long can oligonucleotides be stored at-20°C?

Long-term storage at -20°C is not adequate because the exclusion of any enzymatic reaction can’t be guaranteed. From our experience, oligonucleotides can be stored between one month and two years depending on their individual properties and their different intended purposes and handling.

What is the best media for the resuspension of oligonucleotides?

Therefore, it is recommended that the media used for resuspension of the oligonucleotides lies in the basic pH range. 2. Nuclease degradation There is a danger of degradation of the oligonucleotides through nuclease activity. This nuclease degradation is hastened by the presence of salts, in particular by two-valent and three-valent ions.