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What is the 96-well plate?

What is the 96-well plate?

96-well plates are designed for High Throughput Screening (HTS), sample storage, cell culture, and DNA extraction involving a large sample size. This product has strong durability, with high temperature and chemical resistance.

What is 96-well plate made of?

96-well plates with transparent bottom are manufactured out of white or black polystyrene, with a transparent bottom made of polystyrene. 96-well plates are available in transparent, white pigmented and black pigmented plates.

How deep is a 96-well plate?

½ AreaPlate Microplates

Well format 96-well
Length (mm) 127.76
Width (mm) 85.48
Well diameter (mm) 5.0
Well depth (mm) 11.0

What are microtiter plates used for?

Microtiterplates are convenient, high-throughput tools for organizing tissue culture, PCR tests (such as HIV screening), or immunological assays such as ELISA, RIA, and FIA.. Other applications for PCR plates include analysis of enzyme reactions and compound identification by colorimetric or spectroscopic analysis.

Who invented 96 well plate?

Simultaneously, in another corner of the world, American inventor John Liner devised a vacuum-based panel with 96 wells, and in 1953, gave the world the first disposable vacuum styrene-based panel. Post-1953, he founded the American Linbro company and began mass production of the 96 well plates.

How do you seed a 96 well plate?

For 96-well plates prepare a cell dilution in a sterile container and use a multichannel pipette. Mix well the cell suspension before loading the wells. I mix thoroughly before starting with a 5 or 10 ml pipette and while seeding I use the multichannel to mix 2-3 times between column seeds.

Can I autoclave 96 well plate?

96 well plates are not autoclavable and they will definitely melt.

What volume can a 96 well plate hold?

Technical features of wells Biomat’s 96 Wells Strip Plates are offered with different Well capacities: 350 μl. 360 μl. 400 μl.

Are all 96 well plates the same size?

The length of the 96-well standard microplate is 127.71 mm x the width 85.43 mm and the height is 14.10 mm (shown in fig….ANSI- /SLAS – Standard 1 to 4 – 2004.

96-Well Standard Plate Dimensions
W 85.43 mm
H 14.10 mm
X1 14.36 mm
Xn 113.36 mm

How many wells are in a microtiter plate?

A microplate typically has 6, 12, 24, 48, 96, 384 or 1536 sample wells arranged in a 2:3 rectangular matrix.

Why is 96 well plate used?

Transparent 96-Well Plates are more widely used, while Black 96-Well Plates are used when there is a specific need to minimize background in fluorescence reading. On the contrary, White 96-Well Plates are used to give maximum reflection and to minimize autofluorescence and autoluminescence.

What is the purpose of a well plate?

noun. A rectangular plate or block containing multiple small wells or cavities, used for analysis, clinical testing, or culture; specifically = microtiter plate .

How much media do you put in a 96 well plate?

Growth Area of Tissue Culture Plates and Dishes

Growth Area (cm2) Media Volume (ml)
12 well 3.8 0.76-1.14
24 well 1.9 0.38-0.57
48 well 0.95 0.19-0.28
96 well 0.32 0.1-0.2

How many cells should be seeded in 96 well plate?

Typically, 20,000 – 75,000 cells are seeded per well in Oris™ and Oris™ Pro 96-well plates, and 2,500 – 10,000 per well in Oris™ Pro 384-well plates. For your first experiment, we recommend testing several different cell seeding densities as described in Appendix 1 in the assay protocol.

How do you Sterilise a 96 well plate?

1 – Throw the liquid culture from the plate in a bucket and add bleach to it. After 30min, throw the liquid in the sink and clean the bucket and the sink. 2 – Place the plate in a bucket and add bleach mix to water to completely fill the plate. Wait 30min for complete decontamination of the plate.

Can I reuse 96 well plates?

These plates are not meant to be reused. In our experience when you proceed to autoclave, and repeat an experiment, the results are not the same, if we make a triplicate of known samples, you can give only one or two reference values.

How much medium is in a 96 well plate?

Useful information for various sizes of cell culture dishes and flasks

Catalog No. Growth medium (mL). Approx. volume
24-well 142475 0.5 to 1.0
48-well 150687 0.2 to 0.4
96-well 167008 0.1 to 0.2

Why do we use 96 well plates?

What is the minimum volume of 96 well plate?

The minimum required volume for 96-well microplates is 100 µL, for a half area 96-well plate is 50 µL and for a 384-well plate is 20-25 µL. Sample sizes below those volumes have to be diluted to at least the required minimum volume with the diluent.

How does 96 well plate prevent evaporation?

Utilize a low evaporation lid to reduce fluid loss, to protect samples from evaporation and contamination consider using a lid with condensation rings. The chimney well design of the 96-well standard plates in combination with the condensation rings substantially reduce the risk of contamination and evaporation.

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