What is SSC in fish?

In biochemistry and molecular biology, the saline-sodium citrate (SSC) buffer is used as a hybridization buffer, to control stringency for washing steps in protocols for Southern blotting, in situ hybridization, DNA Microarray or Northern blotting.

What is SSC in situ hybridization?

SSC is used in hybridization cocktails containing DNA and RNA probes for the analysis of unknown sequences and to locate desired genes and gene fragments. SSC is also used to control stringency for washing steps during in situ hybridization. Packet of salts yields 250mL of 20X SSC buffer.

Is SSC a salt?

For In Vitro Diagnostic Use SSC Wash Buffer is a saline sodium citrate buffer suitable for use in in situ hybridization procedures.

What is SSC in Southern blotting?

Saline Sodium Citrate (SSC) is used as a hybridization buffer or a wash buffer in Southern blotting, in situ hybridization, DNA microarrays, or Northern blotting. These products are powders for preparing SSC buffers; each individual powder-containing pouch yields 1 L of SSC buffer, pH 7.0 (2X or 20X).

What is 10X SSC?

Product Description. Saline sodium citrate stock solution (10X, pH 7.0) contains a concentrated solution of filtered saline sodium citrate (SSC) buffer. WARNING This product is not for human or veterinary use.

What does dextran sulfate do in FISH?

Dextran sulfate is considered the most suitable volume exclusion agent for in situ hybridization because of its ability to significantly accelerate hybridization kinetics, while at the same time retaining low levels of non-specific signals.

Why SSC buffer is used?

In biochemistry and molecular biology, saline-sodium citrate (SSC) buffer is used as a hybridization buffer, to control stringency for washing steps in protocols for Southern blotting, in situ hybridization, DNA Microarray or Northern blotting.

What is 20X SSC buffer?

SSC Buffer, 20X, Molecular Grade (pH 7.0), is commonly used in nucleic acid hybridization techniques at concentrations from 0.1X to 20X, depending on the application. Form: Clear, colorless liquid. Composition: 3M NaCl, 0.3M sodium citrate (for 20X concentration).

What is dextran used for?

Dextran is a medication used in managing and treating various clinical conditions, including during hemorrhage, shock, surgical procedures, radiological imaging, antithrombotic administration, and ophthalmic relief of xerophthalmia.

What is dextran sulfate used for?

Dextran sulfate has been used with polyethylene glycol in aqueous biphasic polymer separations for bacteria, virus, proteins, and nucleic acids. The effects on cell proliferation have been studied. It has been shown to form insoluble complexes with fibrinogen.

How do you administer dextran?

This medicine is given through a needle placed in a vein or as a shot into a muscle (usually in the buttock). You may be given a small test dose of this medicine to make sure you or your child are not allergic to it. The rest of your dose will be given at least one hour after the test dose.

Why is dextran used?

Is dextran A IV fluid?

Dextran 40 is a sterile, nonpyrogenic preparation of low molecular weight dextran (average mol. wt. 40,000) in 5% Dextrose Injection or 0.9% Sodium Chloride Injection. It is administered by intravenous infusion.

What is dextran 40 used for?

LMD (dextran 40) is indicated for use in the adjunctive treatment of shock or impending shock due to hemorrhage, burns, surgery or other trauma. It is not indicated as a replacement for whole blood or blood components if they are available.

What is the difference between dextran 40 and 70?

Dextrans have high water-binding capacity; for example, 1 g of dextran 40 retains 30 mL of water, while 1 g dextran 70 retains 20–25 mL of water. Dextran is mostly eliminated by the kidneys and should be avoided in patients with impaired renal function.