What are HMEC?

Product Overview. Lonza’s Human Mammary Epithelial Cells (HMEC) are isolated from adult female breast tissue. The cells have been primarily utilized to understand the formation of breast cancer.

What type of cells are mammary cells?

The mammary epithelium consists of two differentiated cell types organized into two cell layers, an inner layer of luminal epithelial and an outer layer of myoepithelial cells in direct contact with the basement membrane (Fig. 1a).

What are mammary epithelial cells?

Putting the definitions together, mammary epithelial cells are cells in the thin layer of tissue that coat and lines the surface of the milk ducts in the breast. There are many ducts within each lobule that converge in the 15 to 20 lobes of the breast on their way to the tip of the nipple.

What is MCF 10A?

MCF 10A is an epithelial cell line that was isolated in 1984 from the mammary gland of a White, 36-year-old female with fibrocystic breasts. This cell line was deposited by the Michigan Cancer Foundation. Homo sapiens, human. Cell type epithelial cell Morphology epithelial Tissue Breast; Mammary gland Disease.

What are HMEC 1 cells?

HMEC-1 specifically bind lymphocytes in cell adhesion assays. Thus HMEC-1 is the first immortalized human microvascular endothelial cell line that retains the morphologic, phenotypic, and functional characteristics of normal human microvascular endothelial cells.

What are microvascular endothelial cells?

In particular, microvascular endothelial cells are located in the smallest vessels of the circulatory system, such as the capillaries. Vascular endothelial cells have an important role in vessel homeostasis and endothelial cell dysfunction can result in multiple diseases, including atherosclerosis.

Where are mammary cells found?

Mammary glands, which are located in the breast overlying the pectoralis major muscles, are present in both sexes, but usually are functional only in the female.

Which embryonic layer of cells does breast tissue develop from?

Mammary glands are derived from the ectoderm and are a defining feature of mammals. The number of glands that develop along the ‘mammary lines’ on either side of the ventral trunk depends on the mammalian species. The mesoderm specifies the position in which glands develop.

What are the two epithelial lineages of the mammary gland?

Closer examination reveals that the mammary gland consists of two main epithelial lineages, the inner luminal lineage and the outer myoepithelial lineage. The luminal lineage can be further subdivided into mature ductal and milk-producing alveolar cells.

What is mcf7 cell line?

1 MCF-7 human adenocarcinoma cells. MCF-7 is a human breast cancer cell line with estrogen, progesterone and glucocorticoid receptors [26]. It is derived from the pleural effusion of a 69-year-old Caucasian metastatic breast cancer (adenocarcinoma) in 1970 by Dr Soule of the Michigan Cancer Foundation, Detroit, MI [27] …

Does MCF-7 express HER2?

Discoveries in breast cancer pathology through experiments using MCF-7 cells. MCF-7 sub-lines demonstrate a wide divergence in the relative expression of ER, PR and HER2.

What are microvascular cells?

What is mammary tissue composed of?

Parenchyma – The mammary gland’s glandular tissue is made up of branching ducts and terminal secretory lobules. There are 15 to 20 lobes, and a lactiferous duct drains each of them. These ducts enlarge to form the lactiferous sinus before they open separately into the nipple.

What is the arterial supply to the breast?

The main sources of blood supply to the breast are the internal thoracic, lateral thoracic, anterior intercostal, and acromiothoracic arteries (Fig. 9). The internal thoracic perforators are found to be the most reliable by most researchers.

What are the 5 stages of breast development?

What breast changes happen at puberty?

Female Breast Developmental Stages
Stage 4	The areola and nipple become raised and form a second mound above the rest of the breast.
Stage 5	Mature adult breast. The breast becomes rounded and only the nipple is raised.

Where are progenitor cells located?

Bone marrow stromal cells found in the epidermis and make up 10% of progenitor cells. They are often classed as stem cells due to their high plasticity and potential for unlimited capacity for self-renewal. Periosteum contains progenitor cells that develop into osteoblasts and chondroblasts.

Do MCF-7 cells have HER2?

Two particularly important members of this large family are the epidermal growth factor receptor (EGFR), which is activated by the epidermal growth factor (EGF), and the human epidermal growth factor receptor-2 (HER2), both present in MCF-7 cells (7).

Do MCF-7 cells express HER2?

In most of the MCF7 subclones that contain high EGFR overexpressing cells, the cells with EGFR levels exceeding 80 000 molecules per cell exhibit only moderately elevated levels of HER2 (mostly in the range of 200 000–600 000 molecules per cell).

What is the morphology of hmec-1?

HMEC-1 exhibit typical cobblestone morphology when grown in monolayer culture, express and secrete von Willebrand’s Factor, take up acteylated low-density lipoprotein, and rapidly form tubes when cultured on matrigel.

How do I get a certificate of analysis for hmec-1 (crl-3243)?

To download a certificate of analysis for HMEC-1 ( CRL-3243 ), enter the lot number exactly as it appears on your product label or packing slip. The certificate of analysis for that lot of HMEC-1 ( CRL-3243) is not currently available online. Complete this form to request this certificate of analysis.

What is the base medium for this cell line?

The base medium for this cell line is MCDB131 (without L-Glutamine). To make the complete growth medium, add the following components to the base medium: To insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt.