How does Hybrid Capture 2 work?

Hybrid Capture 2 (HC2) technology detects RNA–DNA hybrids using signal-amplified, chemiluminescent technology. Hybrid Capture 2 delivers the accuracy and flexibility necessary for routine detection of DNA that can assist in the diagnosis of infectious diseases.

What is the principle of hybrid capture system?

Principle of Hybrid Capture (HC) HPV Detection Antibodies conjugated with alkaline phosphate and specific for DNA-RNA hybrids react with hybrids which are then subsequently detected using a chemiluminescent substrate. The signal amplification occurs as several conjugated antibodies bind to each hybrid.

What is Hybrid Capture assay?

The HC2 High-Risk HPV DNA Test using Hybrid Capture®2 (HC2) technology is a nucleic acid hybridization assay with signal amplification using microplate chemiluminescence for the qualitative detection of thirteen high-risk types of human papillomavirus (HPV) DNA in cervical specimens.

How does targeted sequencing work?

Targeted sequencing uses deep sequencing to detect known and novel variants within your region of interest. This method generally requires less sample input and produces a smaller amount of data than WGS, making analyses more manageable.

Is PCR used for HPV?

Abstract. Human papillomavirus (HPV) is an etiologic agent of cervical cancer and is the most common sexually transmitted disease in women. PCR amplification of HPV genomes is the most sensitive method for the detection of cervicovaginal HPV.

What is Digene testing?

The digene HC2 High-Risk HPV DNA Test is an in vitro nucleic acid hybridization assay with signal amplification and chemiluminescence for the qualitative detection of 13 types of human papillomavirus (HPV) in cervical specimens. The test is suitable for high- or low-volume testing.

Is HPV test a PCR test?

The system is totally automated and can process 1-30 samples per run and 120 results per patient per day, including genotyping. The Xpert HPV test is a real-time PCR that simultaneously detects DNA encoding for E6/E7 oncoproteins of 14 HPV types (HPV 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66 and 68).

What is a PCR test for HPV?

Background: The development of a reproducible, sensitive, and standardised human papillomavirus (HPV) polymerase chain reaction (PCR) test is required to implement HPV testing in cervical cancer screening programmes and for triaging women with mild to moderate dysplasia.

What is the cheapest sequencing method?

Considerations for DNA sequencing Currently, second-generation NGS technologies are the most commonly used approach because they remain the fastest and the cheapest form of gene sequencing.

What is PCR in a Pap smear?

Human papillomavirus (HPV) with Genotyping polymerase chain reaction (PCR): Negative for HPV genotypes 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, and 68. ThinPrep Pap Test: Satisfactory for evaluation. Negative for intraepithelial lesion or malignancy.

What is HPV other high-risk types PCR?

High-risk HPV types genotyping PCR tested by fluorescence PCR technology, in which 13 kinds of high-risk HPV subtypes were detected, including HPV16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59 and 68.

What is HPV DNA test?

test) A laboratory test in which cells are scraped from the cervix to look for DNA of human papillomaviruses (HPV). HPV can cause abnormal tissue growth (for example, warts) and other changes to cells. Infection for a long time with certain types of HPV can cause cervical cancer.

Can you test for HPV at home?

A person can test themselves for human papillomavirus (HPV) with a home HPV test. Studies suggest that home HPV tests are as accurate as tests that doctors provide, but they may not test for all strains of HPV. Sex and gender exist on spectrums.

How long does it take to repair the hybrid capture 2?

Instrument repair service for the Hybrid Capture 2 System at regional repair center. Shipping, labor and parts costs included during the Full Agreement period. Loaner instrument provided within 2–3 business days. Instrument repair turnaround time of 7–10 business days.

What is the hybrid capture 2 modular system?

The Hybrid Capture 2 Modular System includes an automated plate washer, a luminometer, microplate heater, rotary shaker, and vortexer to automate some of the steps of manually processing Hybrid Capture 2 assays. The Hybrid Capture 2 Modular System provides solutions to manual processing of the HC2 assays.

What is included in the hybrid capture 2 preventive subscription?

One on-site Preventive Maintenance or Inspection Service visit for the Hybrid Capture 2 System, including travel, labor and parts. Includes a 10% discount on repair services during the Preventive Subscription period.

What are the components of the hybrid capture system?

The system consists of the Rotary Shaker I, Microplate Heater I, DML instrument, Automated Plate Washer, Multi-Specimen Tube (MST) Vortexer 2, and customer-configured PC preinstalled with Hybrid Capture Software. More information about customer-configured PC options is available by calling customer service.