What tree does hematoxylin come from?

logwood tree
Hematoxylin is a basic dye derived from the heartwood of Palo de Campeche ( Haematoxylum campechianum), the logwood tree native to Mexico and Central America.

What are the types of haematoxylin?

Table of significant formulations

Formula name	Reference	Mordant
Mayer’s Haematoxylin	Mayer, 1903	Potassium or Ammonium alum
Harris’s Haematoxylin	Harris, 1900	Potassium alum
Cole’s Haematoxylin	Cole, 1943	Potassium alum
Carazzi’s Haematoxylin	Carazzi, 1911	Potassium alum

Can I use expired hematoxylin?

Use of expired hematoxylin impacts staining intensity, i.e., hematoxylin staining intensity decreased as the age of the expired reagents increased.

What are the 3 types of special stains?

Special Stains

• Connective Tissue Stains.
• Microrganisms.
• Carbohydrate Stains.
• Pigments, Minerals and Cytoplasmic Granules.
• Fat Stains.

What is logwood used for?

Logwood was once an important source of black dye, which is obtained from the red heartwood and is still used as a source of the histological stain hematoxylin. The plant is also used in certain traditional systems of medicine.

What is hematoxylin made of?

Hematoxylin is a basic dye derived from the heartwood of Palo de Campeche (Haematoxylum campechianum), the logwood tree native to Mexico and Central America.

What is the pH of hematoxylin?

The pH and peak of absorbance of the aliquots were pH = 2.0 450 NM, 2.5 505, 2.6 507, 2.7 515, 2.8 520, 2.9 530, 3.0 540, 3.1 550, 3.3 560, 3.5 560. In the stained material in the intensity of nuclear staining was about the same at all pH levels but non-specific staining was greatest in slides stained at pH = .

What colour is hematoxylin?

blue-purple color
Hematoxylin has a deep blue-purple color and stains nucleic acids by a complex, incompletely understood reaction. Eosin is pink and stains proteins nonspecifically. In a typical tissue, nuclei are stained blue, whereas the cytoplasm and extracellular matrix have varying degrees of pink staining.

How do you store hematoxylin?

Store reagents at room temperature (18-26°C) protected from light. Reagents are stable until the expiration date shown on the label.

Is hematoxylin acidic or basic?

Hematoxylin is a compound extracted from the heartwood of the logwood tree. Haematoxylin can be considered as a basic dye. It is used to stain acidic structures a purplish blue.

What does hematoxylin stain?

Hematoxylin precisely stains nuclear components, including heterochromatin and nucleoli, while eosin stains cytoplasmic components including collagen and elastic fibers, muscle fibers and red blood cells.

What color is logwood?

purple
Known for its vivid purple shades, logwood quickly became a coveted color for the wealthy. Logwood chips are from the dense heartwood of the logwood tree, which grows in Central America and parts of the West Indies.

How is logwood dye made?

Logwood (Haematoxylum campechianum) The dye logwood or blackwood is extracted from the heartwood of logwood trees (Haematoxylum or Haematoxylon campechianum) that come from Central America. Logwood dye was introduced into Europe by the late 1500s in the form of logs, hence its common name.

What is hematoxylin used for?

Hematoxylin is used to illustrate nuclear detail in cells. Depth of coloration is not only related to the amount of DNA in the nuclei but also to the length of time the sample spends in hematoxylin. Hematoxylin is a reasonably simple dye to make. The dye itself is extracted from the tree Haematoxylum campechianum.

What Colour is hematoxylin?

What color is hematoxylin?

deep blue-purple color

How Lightfast is logwood?

Logwood has good washfastness but moderate lightfastness – a bit of iron improves the lightfastness dramatically.

How do you make logwood dye blue?

Logwood Grey Proceed to make a dye bath as before, with 20%WOF logwood powder and dye your fabrics. By shifting the Ph to alkaline with 1 teaspoon of soda ash per liter of water you will get a more steel blue grey, keep the Ph 5.5 for middle grey.

What is logwood dye?

What color does logwood make?

deep purple
Logwood yields a rich, deep purple. Therefore, it was used as a base or “bottom” for the desirable dark purple and black colors of European fashion and aristocracy. It was in such high demand that in the 18th century, so nearly all black dyed cloth was colored from Logwood.