What are HL-60 cells used for?

HL-60 cells are promyeoloblasts isolated from the peripheral blood by leukopheresis from a 36-year-old, Caucasian female with acute promyelocytic leukemia. This cell line can be used in immune disorder and immunology research. Discounts are available for our fellow nonprofit organizations.

How do you tell the difference between HL and 60 cells?

When needed, neutrophil-like cells can be derived from HL-60 cells through differentiation with DMSO or retinoic acid (3). Differentiated HL-60 cells (dHL-60) can then be used in chemotaxis assays and to visualize the cytoskeleton of a polarized cell (4).

Are HL-60 cells stem cells?

These observations indicate that HL-60 stem cells are multipotent and capable of spontaneous commitment to single-lineage progenitors.

Where did HL-60 cells come from?

HL60 is a human cell line derived from peripheral blood lymphocytes of a 36 year old woman suffering from acute promyleocytic leukaemia. It was one of the first human myeloid leukaemia cells to be established as a continuous suspension cell culture which had previously been difficult to achieve.

What are HL-60 cells and why are they interesting in the study of apoptosis?

The HL-60 cell line has proven useful in the study of cell formation and differentiation of normal and of course leukemic cells. This is because HL-60 cells can be differentiated through the myeloid pathway to cells such as neutrophils quite easily using DMSO, DMF and other inducers such as retinoic acid.

What is Myeloblast?

Listen to pronunciation. (MY-eh-loh-blast) A type of immature white blood cell that forms in the bone marrow. Myeloblasts become mature white blood cells called granulocytes (neutrophils, basophils, and eosinophils).

Why are they called Jurkat cells?

Jurkat cell line was established from the peripheral blood of a 14-year-old boy with acute lymphoblastic leukemia (ALL) at first relapse in 1976; often this cell line is called “JM” (JURKAT and JM are derived from the same patient and are sister clones).

What are SMMC 7721 cells?

The SMMC-7721 cell line was derived from an Asian male patient with hepatocellular carcinoma in 1977. Human hepatoma cell line SMMC-7721 expresses EGFR and VEGFR and is an invaluable tool in liver cancer research.

What is the difference between myeloblast and Lymphoblast?

The main difference between myeloblast and lymphoblast is that the myeloblast differentiates into granulocytes whereas the lymphoblast differentiates into lymphocytes. Furthermore, myeloblast contains granules while lymphoblast does not contain granules.

Is myelocyte and myeloblast the same?

myeloblast, immature blood cell, found in bone marrow, that gives rise to white blood cells of the granulocytic series (characterized by granules in the cytoplasm, as neutrophils, eosinophils, and basophils), via an intermediate stage that is called a myelocyte.

Why are Jurkat cells used?

Jurkat cell model can simulate the function T lymphocytes, so it is widely used in the in vitro studies of T cell signal transduction, cytokines, and receptor expression, and can provide reference and guidance for the treatment of various infectious diseases and the research on their pathogenesis.

What is the history of HL60 cells?

The HL60 cell line was established in 1977 from a patient with acute myeloid leukaemia. The cells largely resemble promyelocytes but can be induced to differentiate terminally in vitro. Some reagents cause HL60 cells to differentiate to granulocyte-like cells, others to monocyte/macrophage-like cell …

What should I look for in HL-60 cells?

I have started working on HL-60 cell lines. My aim is to look for Calcium flux upon activation, chemotaxis and also infection with bacteria. I followed Dr. Steven Ackerman’s protocol for thawing and made sure there is no spontaneous differentiation by checking surface expression of CD11b (Mac-1) staining.

What causes HL60 cells to differentiate to granulocytes?

Some reagents cause HL60 cells to differentiate to granulocyte-like cells, others to monocyte/macrophage-like cell … The HL60 cell line was established in 1977 from a patient with acute myeloid leukaemia. The cells largely resemble promyelocytes but can be induced to differentiate terminally in vitro.

What is the concentration of dimethyl sulfoxide in HL-60 cell culture?

If dimethyl sulfoxide at 1–1.5% is provided for HL-60 cell culture, the cells will differentiate into granulocyte-like cells, or neutrophils. Chemotaxis is one of the most important characteristics of neutrophils in inflammatory response.